LOCUS       40924_49102        3969 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD018, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..855
                     /note="S7942NS1-RA; right arm of the homologous
                     recombination site NS1 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U30252"
     rep_origin      892..1480
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1666..1806)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2025..2471)
                     /gene="ndk"
                     /label=ndk
                     /note="Nucleoside diphosphate kinase from Synechococcus 
                     elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805). 
                     Accession#: P50590"
     misc_feature    2477..2482
                     /label=XbaI
                     /note="XbaI"
     misc_feature    2664..2669
                     /label=NheI
                     /note="NheI"
     misc_feature    2724..2729
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    2759..2764
                     /label=NheI
                     /note="NheI"
     misc_feature    2765..2785
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2783..2788
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(2912..3769)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3770..3874)
                     /label=AmpR promoter
     misc_feature    3900..3905
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagcgacgt cgggggcccc
       61 cggggggatt ctagattctg gagcaggaag atgtcgcggg cattagcacc agcggtctgc
      121 caagcctccg ccagccgttg ggtcccttcc gcttgagctt ttccatcttc gacgatacgg
      181 gcggcggccc cccgcgcttc cgcgatcgcc cgtttacaag ctgcctcagc tggggcgatc
      241 acatcggctt gaagttgctg ctgcacctgt ttgatccgct cctgctgcac agggagttct
      301 gcttggctac gagcgacttc ggtagcaatg tccgcttcag cttcggccac caccgcttcg
      361 cgccgcgtca acgcatcctg aatccggcgc tcggcctcgg cttgggcgat cgctacatcg
      421 cgatcgatcc gacgcagggc cgtgatcttg tcattttcgg ccgtttggat cgcagaggca
      481 gcctgggcat cggcttcagc aattcgggca tctcgctgca gatcagcccg ctgcttgcgt
      541 ccactagccg agagataacc gacctcatcg gaaatgttct ggacttgcag cgtatcgagg
      601 actagaccca gctgctcaag gtcatcctcc gcctcttcca gcagactttt ggcaaaggca
      661 attttgtcct cgttgatctg ctccggcgtg aggctggcta aaacaccacg caagttgcct
      721 tcgagggtct ccttggcaat ttgctcgatt tccttacggt ttttgccaag cagccgctcg
      781 atcgcgttgt ggatggtcgg ttcttcccca gcaatcttga tattggcaac gccttcaaca
      841 gtcaggggaa tgccgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1021 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1561 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1621 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     1681 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     1741 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     1801 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1861 gcatccgctt acagacaagc tccgcggcgg agcgctgctt tcttggcaag cggtcgccag
     1921 ccccaacgcc agggctgcca gcccgaaaca gcggggcaag gcagcttgga agggcgatcg
     1981 cagcacgggc atggcaatgt ctctctgaag gaatgcagac cttattcgta cagccagggt
     2041 tgaatcgtgg gggtccaatc acttagctct gctgggctaa accagagagc aatttcctgt
     2101 tgtgctgttt cgattgcatc cgagccatgg atgatgttgc ggccaatatt gacaccaaaa
     2161 tcaccacgga tggtgcccgg ttctgccgtc agcggattgg tagcgccgat caacttgcga
     2221 gcagccgcca caacgccttc gccttccaag acgatcgcca cgatcggccc agaggtgatg
     2281 aactcgacga ggccattgaa gaaggggcgc tcgcggtgga cagcatagtg ctgttcggcc
     2341 agctcgcgac tgggcttcag ctgctttagg cccaccagtt tgaagccttt ttgctcaaag
     2401 cggccgatga tcgtaccgac caaaccccgc tgaacgccat cgggcttgat ggcaataaat
     2461 gtgcgttcca cagacatcta gatagtcctc aagacgaggc aagcattgag cttgccttcc
     2521 tatggttcgg gatcactggg attcttgaca agcgatcgcg gtcacatcgc tatctcttag
     2581 gacttcgcag cgggcgagtc ggattgaccc ggtagggatt tcgccagatc aatgcccgtg
     2641 gtttgtttca gcttctccag caagctagcg atttgggtag cgctgccttc cccttcgcca
     2701 atcacagtga tcgactccac gtcgatatct ggcacggtgc ctgaaagcgt gacgagcagc
     2761 tagcatcgcg cgcgcgcgcg cggacgtcct cacgttaagg gattttggtc atgagattat
     2821 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2881 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2941 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3001 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3061 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3121 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3181 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3241 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3301 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3361 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3421 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3481 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3541 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3601 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3661 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3721 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3781 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3841 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3901 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3961 cctttcgtc
//
